The content of caffeic acid,salvianolic acid E,rosmarinic acid,lithospermic acid,salvianolic acid B,and salvianolic acid Y within the salvianolic acids for shot was also determined. The linear relationship between chemical compounds was great( r>0. 998 9),and the injection accuracy RSD had been 0. 30%-0. 90%. The intimate RSD is between 1. 4% and 3. 0%,and the RSD of the reproducibility for the plant is between 2. 1% and 5. 2%. The recovery rate regarding the three elements in S. miltiorrhiza had been 96. 80%-99. 20%,and the recovery rate of this Nimodipine cost six components in salvianolic acids for shot was 88. 90%-107. 5%. The perfect solution is of S. miltiorrhiza and salvianolic acids for injection were stable within 48 h. A total of 8 batches of S. miltiorrhiza and injection had been dependant on the research extract,and the difference was smaller than that measured because of the monomer control. This study preliminarily validated that the salvianolic acids guide plant can be utilized as an alternative for the monomer control for the quality-control of S. miltiorrhiza and salvianolic acids for injection.This study is designed to establish a technique when it comes to dedication of As B,As C,DMA,As( Ⅲ),MMA and As( Ⅴ) through the use of HPLC-ICP-MS. A Dioncx Ion PacTMAS7( 4 mm×250 mm) column was useful for the HPLC-ICP-MS technique. The mobile period ended up being 100 mmol·L-1 ammonium carbonate-1. 5 mmol·L-1 ammonium dibasic phosphate( gradient elution) at a flow price of 1 m L·min-1. The shot volume was 10 μL. The linear connections of As B,As C,DMA,As( Ⅲ),MMA,As( Ⅴ) were good aided by the concentration of10-500 μg·L-1. The average data recovery rates( n = 6) had been 105. 7%,100. 5%,102. 9%,105. 7%,100. 2%,92. 69%. The RSD were0. 50%,2. 4%,0. 93%,1. 3%,0. 89%,1. 5%. The precision and repeatability for this technique had been great. In this study,six kinds of arsenic had been divided efficiently by this process. With methodological validation and sample dedication,this method can be used to Mycobacterium infection figure out the morphological valence of arsenic in content determination.Artemisiae Argyi Folium,the dried leaves of Artemisia argyi,has been extensively utilized in standard Chinese and people medicines for a long period. Qiai is among the top-geoherb of Artemisiae Argyi Folium. Trying to investigate dynamic changes of chemical aspects of Qiai in numerous harvest durations and explore the optimum harvest time of Qiai,in this study,the contents of total flavonoids and complete phenolic acids of 36 batches of Qiai accumulated in 6 various harvest periods were reviewed by ultraviolet-visible spectrophotometry. Furthermore,an HPLC method ended up being requested multiple dedication of eight bioactive compounds including six phenolic acids( 5-caffeoylquinic acid,3-caffeoylquinic acid,4-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid) as well as 2 flavonoids( jaceosidin and eupatilin) in Qiai samples. The quantitative outcomes indicated that there have been some differences in the articles of total flavonoids,total phenolic acids and bioactive compounds of Qiai examples in numerous collect times. The dynamic changes of complete flavonoids and total phenolic acids of Qiai in different collect periods had been constant. The items of complete flavonoids and complete phenolic acids of Qiai examples were higher within the third harvest period( across the Dragon Boat Festival),which is basically consistent with the standard harvest times. This current study can provide the basis for determining intestinal microbiology the best collect period of Qiai,and may be helpful for the quality evaluation of this organic medicine.This research was completed to analyze the additional metabolites of endophytic fungi Aspergillosis fumigatus from Euphorbia royleana. The endophytic fungi A. fumigatus had been fermented by solid fermentation,and purified by numerous chromatographic practices after extraction. The frameworks regarding the compounds were identified by1 H-NMR,13 C-NMR and HSQC,HMBC spectra and physicchemical properties. Three substances had been isolated and their frameworks were identified as 3-( 3,4-dihydroxybenzoyl)-5-( 3,4-dihydroxyphenyl)-6-methyl-5,6-dihydro-2 H-pyran-2-one( 1),hydroxysydonic acid( 2) and 11-hydroxysydonic acid( 3). Substance 1 is a unique compound.By preparing 10 batches regarding the content reference of Linggui Zhugan Decoction,the methodology of the characteristic spectral range of the material guide was made. The creaming rate range,the items and the transfer rate number of cinnamaldehyde,glycyrrhizin and glycyrrhizic acid,the characteristic peaks while the similarity number of the characteristic spectrum of Linggui Zhugan Decoction had been determined to make clear crucial quality qualities associated with material reference of Linggui Zhugan Decoction. Into the 10 batches associated with content reference of Linggui Zhugan Decoction,the similarity of characteristic spectrum ended up being more than 0. 9. moreover,after summarizing the characteristic top information,we understood that Fuling had two characteristic peaks,Guizhi had six characteristic peaks,Baizhu had two characteristic peaks and Gancao had 11 characteristic peaks. The average creaming rate of this content reference of this ten batches had been( 12. 13 ± 0. 35) percent. The common content of cinnamaldehyde was 0. 32%,the average transfer price was 10. 69%,the content of cinnamaldehyde in the different batches had been between 0. 22% and 0. 42%,and the transfer rate was between 7. 48% and13. 90%. The average content of glycyrrhizin was 0. 84%,the average transfer price was 50. 39%,the content of glycyrrhizin when you look at the different batches had been between 0. 42% and 1. 26%,and the transfer price ended up being between 35. 27% and 65. 51%. The common content of glycyrrhizic ended up being 1. 88%,the typical transfer price ended up being 40. 74%,the content of glycyrrhizic in the various batches had been between 0. 94% and2. 82%,and the transfer price had been between 28. 52% and 52. 96%. In this paper,the quality value transmitting of material benchmarks of Linggui Zhugan Decoction had been reviewed because of the mix of characteristic spectrum,creaming price therefore the content of list component.
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